|
KMID : 0613820010110040340
|
|
Journal of Life Science
2001 Volume.11 No. 4 p.340 ~ p.345
|
|
|
Embryogenesis and Regeneration from Anther Cultures of Bupleurum falcatum L
|
|
Kwon Soon-Tae
Jeong Hyung-Jin
Kim Kil-Ung
|
|
|
Abstract
|
|
|
|
Effects of growth regulators(2,4-D, IAA, NAA, BA and kinetin) and chilling treatment on callus induction. embryogenesis and regeneration through anther cultures of B. falcatum L. were examined. Frequency of callus induction and embryogenesis was effectively increased by the treatment of chilling at 5^{circ}C for 10 days before anther inoculation. Optimal level of growth regulator for callus induction and embryogenesis from anther was 2,4-D 1.0 mg/L in Murashige and Skoog(MS) basal medium supplemented with 30 g/L sucrose, 8 g/L agar. Frequency of embryogenesis from anther derived callus was increased up to 48% or 45% by addition of IAA 0.1+ kinetin 1.0 mg/L of IAA 0.1+ BA 1.0 mg/L in MS medium, respectively, Optimal medium for obtaining green callus was MS basal supplemented with BA 1.0mg/L. Addition of auxins(IAA or NAA) inhibited the formation of green callus from anther derived callus.
|
|
|
KEYWORD
|
|
|
anther culture, embryogenesis, growth regulatores, callus
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|